5DIR

membrane protein at 2.8 Angstroms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9824
Spacegroup name	C 1 2 1
Unit cell lengths	112.480, 105.880, 85.390
Unit cell angles	90.00, 96.92, 90.00

Refinement procedure

Resolution	44.900 - 2.800
R-factor	0.2207
Rwork	0.219
R-free	0.24500
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	1.025
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (dev_1894)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.900	44.900	2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.153	0.078	0.789
Rpim	0.086	0.043	0.542
Total number of observations	86063	1039	4643
Number of reflections	24069
<I/σ(I)>	7.3	20.7	1.5
Completeness [%]	98.1	92.6	95.6
Redundancy	3.6	3.9	2.7
CC(1/2)	0.984	0.986	0.424

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.8	293	PEG 400, ammonium phosphate