5DIH
Structure of Haliangium ochraceum BMC-T HO-5812
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.628, 66.378, 122.251 |
| Unit cell angles | 90.00, 92.40, 90.00 |
Refinement procedure
| Resolution | 31.508 - 2.444 |
| R-factor | 0.2351 |
| Rwork | 0.233 |
| R-free | 0.27740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fch |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.597 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.508 | 2.580 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.107 | 0.417 |
| Number of reflections | 74297 | |
| <I/σ(I)> | 8.3 | 3.1 |
| Completeness [%] | 96.5 | 93.3 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 298 | 30 mM citric acid/70 mM BIS-Tris propane, 8% PEG 3,350 |
