5DI9

Crystal Structure of hRio2 NES Reverse Mutant Peptide in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2015-02-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	106.692, 106.692, 304.497
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.721 - 2.280
R-factor	0.1694
Rwork	0.168
R-free	0.21150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hb2
RMSD bond length	0.006
RMSD bond angle	0.835
Data scaling software	HKL-2000
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.320
High resolution limit [Å]	2.280	6.190	2.280
Rmerge	0.090	0.025	0.971
Rmeas	0.092	0.027
Rpim	0.035	0.010	0.386
Total number of observations	557247
Number of reflections	127043
<I/σ(I)>	8.3
Completeness [%]	98.0	93.4	99.2
Redundancy	7	6.7	7
CC(1/2)		0.999	0.776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	298	17% PEG3350, 100mM Bis-Tris pH6.4, 200mM NH4NO3, 10mM Spermine HCl