5DI3

Crystal structure of Arl13B in complex with Arl3 of Chlamydomonas reinhardtii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.916
Spacegroup name	P 21 21 21
Unit cell lengths	57.100, 68.800, 120.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.843 - 2.500
R-factor	0.2009
Rwork	0.199
R-free	0.23640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bh6
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.600
High resolution limit [Å]	2.500	5.000	2.500
Rmerge	0.065	0.034	0.628
Rmeas	0.071	0.038	0.680
Total number of observations	109004
Number of reflections	16944	2239	1840
<I/σ(I)>	17.56	37.46	3.26
Completeness [%]	99.9	99.3	99.9
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Tris pH 8.5, 25 % PEG 6000