5DHY
HIV-1 Rev NTD dimers with variable crossing angles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2010-07-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 4 |
| Unit cell lengths | 121.140, 121.140, 89.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.390 - 3.100 |
| R-factor | 0.2919 |
| Rwork | 0.291 |
| R-free | 0.30020 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.140 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.800 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 11655 | |
| <I/σ(I)> | 15.1 | 2.7 |
| Completeness [%] | 99.8 | 97.6 |
| Redundancy | 7.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Crystals of scFv-Rev were initially grown in 20% PEG 3350, a variety of salts (200 mM sodium sulfate, sodium bromide, or ammonium phosphate dibasic), and pH ranging from 6.5-8.5 |
