5DHF
Crystal Structure of hRio2 NES Peptide in complex with CRM1-Ran-RanBP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2015-02-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 106.479, 106.479, 303.731 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.718 - 2.285 |
R-factor | 0.1791 |
Rwork | 0.178 |
R-free | 0.21850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hb2 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.617 |
Data scaling software | SCALEPACK |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.320 |
High resolution limit [Å] | 2.280 | 6.190 | 2.280 |
Rmerge | 0.073 | 0.025 | 0.890 |
Rmeas | 0.078 | 0.027 | 0.972 |
Rpim | 0.028 | 0.010 | 0.376 |
Total number of observations | 552057 | ||
Number of reflections | 79060 | ||
<I/σ(I)> | 9 | ||
Completeness [%] | 98.6 | 93.4 | 99.8 |
Redundancy | 7 | 6.6 | 5.9 |
CC(1/2) | 0.997 | 0.701 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 298 | 17% PEG3350, 100mM Bis-Tris pH6.4, 200mM NH4NO3, 10mM Spermine HCl |