5DHC
Cooperativity and Downstream Binding in RNA Replication
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2015-04-21 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 3 |
| Unit cell lengths | 43.107, 43.107, 83.245 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.550 |
| R-factor | 0.17164 |
| Rwork | 0.170 |
| R-free | 0.19501 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nfo |
| RMSD bond length | 0.023 |
| RMSD bond angle | 3.008 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.096 | 0.461 |
| Number of reflections | 25146 | |
| <I/σ(I)> | 17.54 | 2.14 |
| Completeness [%] | 100.0 | |
| Redundancy | 2.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 10% MPD, sodium cacodylate, spermine tetrahydrochloride, strontium chloride |
