5DH7
Two divalent metal ions and conformational changes play roles in the hammerhead ribozyme cleavage reaction-G12A mutant in Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-15 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.8923 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 81.309, 85.825, 103.528 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.655 - 3.064 |
R-factor | 0.2053 |
Rwork | 0.200 |
R-free | 0.25220 |
RMSD bond length | 0.001 |
RMSD bond angle | 0.376 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.130 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.135 | 0.252 |
Number of reflections | 12239 | |
<I/σ(I)> | 26.5 | 6 |
Completeness [%] | 92.9 | 70.2 |
Redundancy | 4.4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 33 % MPD, 0.4 M potassium chloride, 50 mM potassium acetate pH 5.0, 10 mM MgCl2, and 0.5mM spermine |