5DH0
Structure of the siderophore periplasmic binding protein from the fuscachelin gene cluster of Thermobifida fusca in P41
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 41 |
| Unit cell lengths | 72.983, 72.983, 135.267 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.359 - 2.444 |
| R-factor | 0.1732 |
| Rwork | 0.171 |
| R-free | 0.21850 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS (OSX10.9.5_Darwin13.4.0) |
| Data scaling software | Aimless (0.5.8) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.360 | 2.540 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.101 | 0.689 |
| Number of reflections | 26118 | |
| <I/σ(I)> | 12.2 | 3.3 |
| Completeness [%] | 99.6 | 97.6 |
| Redundancy | 6.2 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.16 M ammonium acetate 32% w/v PEG 4,000 0.1 M MES pH 6.5 |
