5DGV
Complex of yeast 80S ribosome with hypusine-containing/non-modified eIF5A and/or a peptidyl-tRNA analog
これはPDB形式変換不可エントリーです。
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.14271 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 437.140, 287.910, 303.850 |
| 格子定数 [度] | 90.00, 98.76, 90.00 |
精密化法
| 残基 | 149.047 - 3.100 |
| R因子 | 0.2389 |
| Rwork | 0.238 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v88 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.018 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 149.047 | 3.200 | |
| 分解能 [Å] (高) | 3.100 | 125.000 | 3.100 |
| Rmerge_l_obs | 0.557 | 0.056 | 1.379 |
| Rmeas | 0.594 | 0.067 | 1.575 |
| Total number of observations | 6878841 | ||
| 独立反射数 | 1338357 | 12 | 121237 |
| <I/σ(I)> | 7.97 | 7.86 | 1.34 |
| 完全性 [%] | 99.9 | 44.4 | 100 |
| 冗長性 | 5.1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277.15 | Tris-acetate pH7.0, potassium thiocyanate, magnesium acetate, glycerol, spermidine, PEG 20000 |
