5DGG
Central domain of uncharacterized Lpg1148 protein from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.085, 56.564, 121.994 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.300 - 1.930 |
| R-factor | 0.1777 |
| Rwork | 0.175 |
| R-free | 0.21810 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.540 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.960 |
| High resolution limit [Å] | 1.930 | 5.240 | 1.930 |
| Rmerge | 0.078 | 0.029 | 0.743 |
| Rmeas | 0.086 | 0.032 | 0.834 |
| Rpim | 0.036 | 0.014 | 0.372 |
| Total number of observations | 145864 | ||
| Number of reflections | 26237 | ||
| <I/σ(I)> | 8.3 | 2.1 | |
| Completeness [%] | 99.9 | 99.2 | 99.3 |
| Redundancy | 5.6 | 5.2 | 4.9 |
| CC(1/2) | 0.999 | 0.742 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 294 | 2 M ammonium sulfate, 4% sucrose, 0.01 M magnesium chloride |
