5DGG

Central domain of uncharacterized Lpg1148 protein from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	49.085, 56.564, 121.994
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.300 - 1.930
R-factor	0.1777
Rwork	0.175
R-free	0.21810
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.540
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.960
High resolution limit [Å]	1.930	5.240	1.930
Rmerge	0.078	0.029	0.743
Rmeas	0.086	0.032	0.834
Rpim	0.036	0.014	0.372
Total number of observations	145864
Number of reflections	26237
<I/σ(I)>	8.3		2.1
Completeness [%]	99.9	99.2	99.3
Redundancy	5.6	5.2	4.9
CC(1/2)		0.999	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	294	2 M ammonium sulfate, 4% sucrose, 0.01 M magnesium chloride