5DGE
Coping with proline stalling: structural basis of hypusine-induced protein synthesis by the eukaryotic ribosome
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.148 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 438.000, 289.050, 305.260 |
| Unit cell angles | 90.00, 98.95, 90.00 |
Refinement procedure
| Resolution | 122.875 - 3.450 |
| R-factor | 0.2083 |
| Rwork | 0.207 |
| R-free | 0.26250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v88 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.131 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 122.875 | 3.500 | |
| High resolution limit [Å] | 3.450 | 100.000 | 3.450 |
| Rmerge | 0.377 | 0.104 | 1.380 |
| Rmeas | 0.408 | 0.140 | 1.583 |
| Total number of observations | 5302502 | ||
| Number of reflections | 981624 | 20 | 41424 |
| <I/σ(I)> | 5.55 | 6.03 | 1.05 |
| Completeness [%] | 99.9 | 40 | 99.9 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277.15 | Tris-acetate pH7.0, potassium thiocyanate, magnesium acetate, glycerol, spermidine, PEG 20000 |
