5DGC
Reaction of phosphorylated CheY with imidazole 2 of 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2014-10-13 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.490, 53.580, 161.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.430 - 1.940 |
| R-factor | 0.1697 |
| Rwork | 0.169 |
| R-free | 0.18890 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.598 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 26.430 |
| High resolution limit [Å] | 1.940 |
| Number of reflections | 36929 |
| <I/σ(I)> | 10.4 |
| Completeness [%] | 99.7 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | MnCl2, BeCl2, NaF, imidazole, Tris, ammonium sulfate, and glycerol |
