5DG7
CRYSTAL STRUCTURE OF HUMAN DNA POLYMERASE ETA INSERTING dTTP ACROSS A DNA TEMPLATE CONTAINING 1,N6-ETHENODEOXYADENOSINE LESION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 61 |
Unit cell lengths | 99.039, 99.039, 81.826 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.885 - 2.260 |
R-factor | 0.1586 |
Rwork | 0.156 |
R-free | 0.21230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o3n |
RMSD bond length | 0.008 |
RMSD bond angle | 1.154 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.165 | 0.893 |
Number of reflections | 21572 | |
<I/σ(I)> | 12.571 | 1.702 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 7.3 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1M MES pH 5.5, 5mM calcium chloride, 16% PEG 2000 MME |