5DFY
Structure of the parental state of GAF3 from Slr1393 of Synechocystis sp. PCC6803 (in vitro assembled protein/chromophore)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00894 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.940, 62.940, 119.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.629 - 1.600 |
| R-factor | 0.1559 |
| Rwork | 0.155 |
| R-free | 0.18060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.322 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.510 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 32428 | |
| <I/σ(I)> | 27.87 | 6.44 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 12.71 | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 285 | 0.02 M sodium citrate pH 5.6, 0.1 M sodium chloride, 11 % (w/v) PEG 3350 |
