5DFT
Structure of the Eleventh Type III Domain from Human Fibronectin
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-03 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.493, 107.402, 210.337 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.902 - 2.500 |
R-factor | 0.1968 |
Rwork | 0.193 |
R-free | 0.24810 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.360 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 32405 | |
<I/σ(I)> | 10.8 | 1.9 |
Completeness [%] | 99.2 | 99.1 |
Redundancy | 4.7 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.1M sodium citrate, 0.1M citric acid and 0.1M sodium iodine | |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.1M sodium citrate, 0.1M citric acid and 0.1M sodium iodine |