5DFN

Structure of Tetrahymena Telomerase P45 C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	F 2 2 2
Unit cell lengths	88.030, 124.620, 173.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	86.885 - 2.382
R-factor	0.2127
Rwork	0.207
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.009
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	86.890	86.890	2.440
High resolution limit [Å]	2.380	10.650	2.380
Rmerge	0.064	0.028	0.728
Rmeas	0.072	0.031	0.814
Total number of observations	101012
Number of reflections	18884	239	1107
<I/σ(I)>	14.07	32.72	2.54
Completeness [%]	97.8	95.6	78.6
Redundancy	5.35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	14 mg/ml P45 protein, 0.1 M Na-HEPES buffer, 5%v/v MPD, 10%w/v PEG-6000