5DFM

Structure of Tetrahymena telomerase p19 fused to MBP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 3
Unit cell lengths	150.600, 150.600, 150.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	86.949 - 2.301
R-factor	0.1799
Rwork	0.175
R-free	0.22570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.143
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	86.949	86.949	2.360
High resolution limit [Å]	2.300	10.290	2.300
Rmerge	0.119	0.038	1.033
Rmeas	0.122	0.039	1.062
Total number of observations	993498
Number of reflections	50601	640	3679
<I/σ(I)>	19.38	51.51	3.43
Completeness [%]	99.9	99.5	98.7
Redundancy	19.63		18.81

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	12 mg/ml MBP-P19 protein, 0.1 M Sodium Acetate, 2.0 M Ammonium Sulfate