5DFK
Crystal Structure of the Escherichia coli Common Pilus Chaperone, EcpB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 62.650, 62.650, 121.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.260 - 2.400 |
| R-factor | 0.20669 |
| Rwork | 0.205 |
| R-free | 0.23774 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.307 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.260 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.057 | 0.492 |
| Number of reflections | 11265 | |
| <I/σ(I)> | 44.4 | 6.6 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 19.2 | 19.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 15% (v/v) glycerol, 15% (w/v) PEG 5000 MME |
