5DEK
RNA octamer containing dT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 44.059, 44.059, 85.398 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.155 - 1.993 |
| R-factor | 0.2411 |
| Rwork | 0.240 |
| R-free | 0.26210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.303 |
| Data reduction software | HKL-2000 (v706e) |
| Data scaling software | HKL-2000 (v706e) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.150 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.126 | 0.081 | 0.682 |
| Rmeas | 0.128 | ||
| Rpim | 0.038 | ||
| Total number of observations | 77578 | ||
| Number of reflections | 10680 | ||
| <I/σ(I)> | 7.5 | 1.8 | |
| Completeness [%] | 97.6 | 97.4 | 96.6 |
| Redundancy | 12.8 | 11.3 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 40 mM sodium cacodylate, 20 mM hexamine Co(III) chloride, 20 mM MgCl2, 80 mM NaCl |
