5DEG
Crystal structure of B*27:06 bound to the pVIPR peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-10 |
Detector | MARRESEARCH |
Wavelength(s) | 1.005 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.768, 81.684, 64.433 |
Unit cell angles | 90.00, 107.53, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.830 |
R-factor | 0.17214 |
Rwork | 0.171 |
R-free | 0.19308 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a83 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.267 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.900 |
High resolution limit [Å] | 1.830 | 1.830 |
Number of reflections | 43847 | |
<I/σ(I)> | 34.7 | 6 |
Completeness [%] | 99.1 | 92.2 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 20%(w/v) PEG 8000, 100 mM Tris-HCl pH 7.0 |