5DE0

Dye-decolorizing protein from V. cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-02
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 32
Unit cell lengths	77.700, 77.700, 156.910
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.296 - 2.240
R-factor	0.2368
Rwork	0.236
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qnr
RMSD bond length	0.003
RMSD bond angle	0.745
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.240	2.240
Rmerge		0.767
Number of reflections	50860
<I/σ(I)>	12.51	2.4
Completeness [%]	99.8	99
Redundancy	5.83	5.72

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	potassium thiocyanate, PEG 3000