5DDQ
L-glutamine riboswitch bound with L-glutamine soaked with Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | FILM |
| Collection date | 2013-05-01 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.605, 85.701, 60.889 |
| Unit cell angles | 90.00, 99.72, 90.00 |
Refinement procedure
| Resolution | 25.772 - 2.400 |
| R-factor | 0.2026 |
| Rwork | 0.197 |
| R-free | 0.25660 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.966 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.400 |
| <I/σ(I)> | 16.7 |
| Completeness [%] | 85.8 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 0.1 M HEPES-sodium, pH 7.0, 40% (v/v) 2-methyl-2,4-pentanediol, 2 mM MnCl2 |
