5DD8

The Crystal structure of HucR mutant (HucR-E48Q) from Deinococcus radiodurans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-23
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979200
Spacegroup name	P 61
Unit cell lengths	44.931, 44.931, 284.825
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.910 - 2.050
R-factor	0.21903
Rwork	0.217
R-free	0.25667
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fbk
RMSD bond length	0.015
RMSD bond angle	1.734
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.910	2.120
High resolution limit [Å]	2.050	2.050
Number of reflections	19997
<I/σ(I)>	2.1
Completeness [%]	99.0	94.1
Redundancy	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	22% (w/v) PEG 3350, 500 mM MgCl2, 100 mM Bis-Tris (pH 7.0)