5DD5

Crystal structures in an anti-HIV antibody lineage from immunization of Rhesus macaques

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2014-05-12
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	38.350, 77.430, 138.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.700 - 1.900
R-factor	0.2018
Rwork	0.198
R-free	0.26050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dd0
RMSD bond length	0.007
RMSD bond angle	1.110
Data reduction software	MOSFLM
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	17.700
High resolution limit [Å]	1.900
Number of reflections	33258
<I/σ(I)>	5
Completeness [%]	99.5
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M Bis-Tris pH 6.5, 20% PEG 1500