5DD1
Crystal structures in an anti-HIV antibody lineage from immunization of Rhesus macaques
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-10-08 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.115, 49.168, 54.653 |
| Unit cell angles | 90.52, 104.58, 96.57 |
Refinement procedure
| Resolution | 21.832 - 1.597 |
| R-factor | 0.1865 |
| Rwork | 0.185 |
| R-free | 0.22830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dd0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.139 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 46323 | |
| <I/σ(I)> | 25.4 | |
| Completeness [%] | 81.3 | 84.3 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 MES, 14% PEG 4000 |
