5DCX
Structural studies of AAV2 Rep68 reveal a partially structured linker and compact domain conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-11-02 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.644, 178.706, 130.358 |
| Unit cell angles | 90.00, 91.66, 90.00 |
Refinement procedure
| Resolution | 29.814 - 2.600 |
| R-factor | 0.1928 |
| Rwork | 0.191 |
| R-free | 0.23750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zo0 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.781 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.810 | 29.810 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.590 | 2.600 |
| Rmerge | 0.075 | 0.038 | 0.311 |
| Total number of observations | 43373 | 41772 | |
| Number of reflections | 105074 | ||
| <I/σ(I)> | 12 | 25.6 | 4.1 |
| Completeness [%] | 99.1 | 96.4 | 98.9 |
| Redundancy | 3.82 | 3.59 | 3.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 50 mM sodium cacodylate, pH 6.0, 90 mM potassium chloride, 20 mM magnesium chloride, 14-17% isopropanol |
