5DCV
Crystal structure of PhoRpp38-SL12M complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-20 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.090, 79.170, 98.470 |
| Unit cell angles | 90.00, 107.40, 90.00 |
Refinement procedure
| Resolution | 32.398 - 3.401 |
| R-factor | 0.2653 |
| Rwork | 0.263 |
| R-free | 0.31130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2czw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.383 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.610 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 1.162 | |
| Number of reflections | 11936 | |
| <I/σ(I)> | 18.85 | 1.39 |
| Completeness [%] | 98.7 | 98.9 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 2-prooanol, Mgnessium accetate, sodium cacodylate |
