5DCP

Crystal structure of the human filamin B Ig-like domains 16-17

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-28
Detector	PSI PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 3 2 1
Unit cell lengths	80.620, 80.620, 118.016
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.340 - 2.490
R-factor	0.208
Rwork	0.206
R-free	0.25020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2k7p
RMSD bond length	0.015
RMSD bond angle	1.681
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.550
High resolution limit [Å]	2.490	11.140	2.490
Rmerge	0.099	0.071	0.579
Rmeas	0.106	0.077	0.622
Total number of observations	129873
Number of reflections	16042	221	1111
<I/σ(I)>	12.79	20.7	3.14
Completeness [%]	99.7	97.8	96.2
Redundancy	12.4		6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	3.5 M Sodium formate