5DCM

Structure of a lantibiotic response regulator: C-terminal domain of the nisin resistance regulator NsrR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-12
Detector	MAR CCD 165 mm
Wavelength(s)	1.07145
Spacegroup name	P 21 21 2
Unit cell lengths	56.380, 60.460, 56.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.850 - 1.600
R-factor	0.1893
Rwork	0.187
R-free	0.21880
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.090
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	1.600
Rmerge	0.056
Number of reflections	124813
<I/σ(I)>	21.6
Completeness [%]	98.8
Redundancy	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		285	0.1 M SPG buffer pH 8.0 and 25% (w/v) PEG 1500