5DCG
Crystal Structure of WT Apo Human Glutathione Transferase Pi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-06-25 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 77.499, 90.250, 68.920 |
Unit cell angles | 90.00, 98.49, 90.00 |
Refinement procedure
Resolution | 38.325 - 2.010 |
R-factor | 0.1497 |
Rwork | 0.148 |
R-free | 0.19000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gss |
Data scaling software | Aimless (0.5.4) |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 68.160 | 68.160 | 2.060 |
High resolution limit [Å] | 2.010 | 8.980 | 2.010 |
Rmerge | 0.116 | 0.076 | 0.224 |
Rpim | 0.059 | 0.041 | 0.114 |
Total number of observations | 149880 | 1742 | 10403 |
Number of reflections | 30673 | ||
<I/σ(I)> | 9.7 | 15.9 | 5.7 |
Completeness [%] | 98.0 | 99.4 | 95.6 |
Redundancy | 4.9 | 4.6 | 4.7 |
CC(1/2) | 0.984 | 0.977 | 0.957 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100MM MES, PH 6.0, 28% (W/V) PEG 8000, 20MM CACL2, 10MM DTT |