5DCF

C-terminal domain of XerD recombinase in complex with gamma domain of FtsK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9184
Spacegroup name	P 65
Unit cell lengths	83.451, 83.451, 88.672
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.135 - 2.300
R-factor	0.1927
Rwork	0.191
R-free	0.22890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a0p 2j5p
RMSD bond length	0.010
RMSD bond angle	1.162
Data scaling software	Aimless (0.2.7)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.020	56.020	2.380
High resolution limit [Å]	2.300	8.910	2.300
Rmerge	0.136	0.052	1.736
Rpim	0.044	0.020	0.540
Total number of observations	169816	2320	17378
Number of reflections	15676
<I/σ(I)>	62	1020.2	2
Completeness [%]	100.0	99.7	100
Redundancy	10.8	8	11.2
CC(1/2)	0.997	0.998	0.531

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	291	Bicine, PEG 8000