5DC4

CRYSTAL STRUCTURE OF MONOBODY AS25/ABL1 SH2 DOMAIN COMPLEX, CRYSTAL A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	61.281, 74.928, 39.229
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.930 - 1.480
R-factor	0.14677
Rwork	0.144
R-free	0.18965
RMSD bond length	0.010
RMSD bond angle	1.205
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.930	1.520
High resolution limit [Å]	1.480	1.480
Rmerge		0.235
Number of reflections	30102
<I/σ(I)>	29.7	4.3
Completeness [%]	98.2	95.1
Redundancy	4.9	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.83	291	0.1M Imidazole pH 7.83 and 3.5M NaCl