5DBO
Crystal structure of the tetrameric eIF2B-beta2-delta2 complex from C. thermophilum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-14 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.85506 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 109.430, 109.430, 219.060 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.948 - 3.000 |
R-factor | 0.2105 |
Rwork | 0.208 |
R-free | 0.25460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ZEM and 4ZEO |
RMSD bond length | 0.007 |
RMSD bond angle | 1.316 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.7) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.180 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 31063 | |
<I/σ(I)> | 14.73 | 2.8 |
Completeness [%] | 99.6 | 98.3 |
Redundancy | 8.21 | |
CC(1/2) | 0.841 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | MES (pH 6.0), pentaerythritol propoxylate (5/4 PO/OH), NaCl |