5DBB

Structure of human DNA polymerase beta Host-Guest complex with the dG base paired with a dA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-07-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5468
Spacegroup name	P 1 21 1
Unit cell lengths	54.389, 79.372, 54.938
Unit cell angles	90.00, 105.45, 90.00

Refinement procedure

Resolution	19.843 - 2.250
R-factor	0.2086
Rwork	0.206
R-free	0.26450
RMSD bond length	0.003
RMSD bond angle	0.642
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.250
Number of reflections	21462
<I/σ(I)>	17.9
Completeness [%]	100.0
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	14% to 23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole (pH 7.5)