5DBB
Structure of human DNA polymerase beta Host-Guest complex with the dG base paired with a dA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-07-15 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5468 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.389, 79.372, 54.938 |
Unit cell angles | 90.00, 105.45, 90.00 |
Refinement procedure
Resolution | 19.843 - 2.250 |
R-factor | 0.2086 |
Rwork | 0.206 |
R-free | 0.26450 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.642 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.250 |
Number of reflections | 21462 |
<I/σ(I)> | 17.9 |
Completeness [%] | 100.0 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 14% to 23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole (pH 7.5) |