5DB9
Structure of human DNA polymerase beta Host-Guest complex with the N7MG base paired with a dG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97468 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.201, 79.278, 54.720 |
Unit cell angles | 90.00, 105.63, 90.00 |
Refinement procedure
Resolution | 19.820 - 2.450 |
R-factor | 0.1905 |
Rwork | 0.187 |
R-free | 0.25970 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.260 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.450 |
Number of reflections | 16411 |
<I/σ(I)> | 13.8 |
Completeness [%] | 99.4 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 14 to 23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole (pH 7.5) |