5DB9

Structure of human DNA polymerase beta Host-Guest complex with the N7MG base paired with a dG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97468
Spacegroup name	P 1 21 1
Unit cell lengths	54.201, 79.278, 54.720
Unit cell angles	90.00, 105.63, 90.00

Refinement procedure

Resolution	19.820 - 2.450
R-factor	0.1905
Rwork	0.187
R-free	0.25970
RMSD bond length	0.009
RMSD bond angle	1.260
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.450
Number of reflections	16411
<I/σ(I)>	13.8
Completeness [%]	99.4
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	14 to 23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole (pH 7.5)