5DB7

Structure of human DNA polymerase beta Host-Guest complex with the N7MG base paired with a dT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97648
Spacegroup name	P 1 21 1
Unit cell lengths	54.396, 79.480, 54.912
Unit cell angles	90.00, 105.36, 90.00

Refinement procedure

Resolution	19.870 - 2.209
R-factor	0.2235
Rwork	0.221
R-free	0.27300
RMSD bond length	0.003
RMSD bond angle	0.855
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.220
Number of reflections	22542
<I/σ(I)>	20.4
Completeness [%]	99.9
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	14% to 23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole (pH 7.5)