5DAT

Complex of yeast 80S ribosome with hypusine-containing eIF5A

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1587
Spacegroup name	P 1 21 1
Unit cell lengths	435.630, 286.450, 303.410
Unit cell angles	90.00, 98.85, 90.00

Refinement procedure

Resolution	189.161 - 3.150
R-factor	0.2046
Rwork	0.204
R-free	0.25210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v88
RMSD bond length	0.013
RMSD bond angle	1.273
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	189.161		3.200
High resolution limit [Å]	3.150	100.000	3.150
Rmerge		0.104
Rmeas	0.408	0.140	1.583
Total number of observations	5302502
Number of reflections	1262028	20	41424
<I/σ(I)>	6.54	6.03	1.03
Completeness [%]	99.9	40	99.6
Redundancy	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	Tris-acetate pH7.0, potassium thiocyanate, magnesium acetate, glycerol, spermidine, PEG 20000