5DAT
Complex of yeast 80S ribosome with hypusine-containing eIF5A
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1587 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 435.630, 286.450, 303.410 |
Unit cell angles | 90.00, 98.85, 90.00 |
Refinement procedure
Resolution | 189.161 - 3.150 |
R-factor | 0.2046 |
Rwork | 0.204 |
R-free | 0.25210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v88 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.273 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 189.161 | 3.200 | |
High resolution limit [Å] | 3.150 | 100.000 | 3.150 |
Rmerge | 0.104 | ||
Rmeas | 0.408 | 0.140 | 1.583 |
Total number of observations | 5302502 | ||
Number of reflections | 1262028 | 20 | 41424 |
<I/σ(I)> | 6.54 | 6.03 | 1.03 |
Completeness [%] | 99.9 | 40 | 99.6 |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277.15 | Tris-acetate pH7.0, potassium thiocyanate, magnesium acetate, glycerol, spermidine, PEG 20000 |