5DAM
Crystal Structure of p-iodoHoechst bound to d(CGCAAATTTGCG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2001-01-25 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 24.638, 40.408, 64.344 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.950 |
R-factor | 0.1783 |
Rwork | 0.178 |
R-free | 0.29720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NDB ID GDL039 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.027 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.057 | 0.226 |
Number of reflections | 4783 | |
<I/σ(I)> | 22.17 | 5.62 |
Completeness [%] | 94.2 | 91.7 |
Redundancy | 4.21 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | HANGING DROP, 19 DEG C |