5DAG
Crystal structure of PZP domain of human AF10 protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.283 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 51.907, 51.907, 147.727 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.869 - 1.600 |
R-factor | 0.192 |
Rwork | 0.190 |
R-free | 0.21430 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.001 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.600 |
Rmerge | 0.075 |
Number of reflections | 22991 |
<I/σ(I)> | 55 |
Completeness [%] | 98.8 |
Redundancy | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Crystals were grown from a solution containing 0.1 M Tris-HCl pH 8.5, 25% PEG3350 using the hanging drop vapor diffusion method |