5DA9
ATP-gamma-S bound Rad50 from Chaetomium thermophilum in complex with the Rad50-binding domain of Mre11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9797 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 116.511, 125.086, 168.304 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.898 - 3.000 |
| R-factor | 0.2182 |
| Rwork | 0.216 |
| R-free | 0.25760 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 1.114 | |
| Number of reflections | 47393 | |
| <I/σ(I)> | 14.83 | 1.58 |
| Completeness [%] | 99.2 | 95.5 |
| Redundancy | 6.9 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M sodium chloride, 0.1 M magnesium chloride, 11% PEG 1500, 0.1 M Hepes buffer |
