5DA8
Crystal structure of chaperonin GroEL from
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 |
| Unit cell lengths | 138.996, 159.781, 228.823 |
| Unit cell angles | 75.46, 90.51, 91.22 |
Refinement procedure
| Resolution | 48.600 - 3.000 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ki8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.380 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.050 | 0.436 |
| Number of reflections | 375234 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 99.0 | 98.5 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 297 | 0.1M Magnesium Chloride, 0.1M Imidazole, 35% MPD |
