5DA1
A Dimerization-Dependent Mechanism Drives PRRSV NSP11 Functions As a Beta Interferon Antagonist and Endoribonuclease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97910 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 77.317, 77.317, 204.693 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.750 |
Rwork | 0.211 |
R-free | 0.28190 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.320 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4-1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.800 | |
Number of reflections | 17000 | |
<I/σ(I)> | 24.4 | 5.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.7 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | sodium citrate tribasic dehydrate, PEG 3350 |