5DA0
Structure of the the SLC26 transporter SLC26Dg in complex with a nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.650, 117.280, 84.360 |
| Unit cell angles | 90.00, 100.09, 90.00 |
Refinement procedure
| Resolution | 19.946 - 3.200 |
| R-factor | 0.2609 |
| Rwork | 0.258 |
| R-free | 0.30800 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.744 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.053 | |
| Number of reflections | 20209 | |
| <I/σ(I)> | 18.7 | 2 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 6.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 1M Ammoniumformate, 50 mM Na Acetate pH 4.5, 48% PEG 400 |
