5D9T
Crystal structure of dehydroascorbate reductase (OsDHAR) from Oryza sativa L. japonica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.030, 48.380, 51.830 |
| Unit cell angles | 90.00, 107.41, 90.00 |
Refinement procedure
| Resolution | 24.728 - 1.900 |
| R-factor | 0.1558 |
| Rwork | 0.154 |
| R-free | 0.19630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.757 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | |
| Number of reflections | 17080 | |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 97.0 | 97.1 |
| Redundancy | 6.8 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 30%(w/v) PEG MME 2000, 0.15M potassium bromide |
