5D9R

Crystal structure of a conserved domain in the intermembrane space region of the plastid division protein ARC6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 31 2 1
Unit cell lengths	60.339, 60.339, 92.326
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.476 - 2.052
R-factor	0.2063
Rwork	0.205
R-free	0.22270
Data scaling software	SCALEPACK
Phasing software	SHELXDE
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.120
High resolution limit [Å]	2.050	4.420	2.050
Rmerge	0.078	0.057	0.399
Rmeas	0.087	0.063	0.442
Rpim	0.037	0.027	0.189
Total number of observations	67102
Number of reflections	12651
<I/σ(I)>	14.2
Completeness [%]	99.8	98.4	99.9
Redundancy	5.3	4.8	5.4
CC(1/2)		0.996	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	295	PEG4000, magnesium sulfate, tri-sodium citrate, isopropanol