5D9G

Crystal structure of TIPRL, TOR signaling pathway regulator-like, in complex with peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.99219
Spacegroup name	P 65
Unit cell lengths	145.307, 145.307, 95.950
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.616 - 2.150
R-factor	0.1832
Rwork	0.182
R-free	0.19850
Structure solution method	MAD
RMSD bond length	0.003
RMSD bond angle	0.691
Data scaling software	Aimless (0.5.1)
Phasing software	SHELX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	95.950	95.950	2.250
High resolution limit [Å]	2.150	7.130	2.150
Rmerge	0.119	0.095	1.574
Rpim	0.031	0.025	0.413
Total number of observations	952365	26797	125665
Number of reflections	62695
<I/σ(I)>	12.1	27.6	2.3
Completeness [%]	100.0	100	100
Redundancy	15.2	14.8	15.1
CC(1/2)	0.995	0.993	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	100mM SPG buffer, pH 5.5. 1.5M NaCl, TIPRL 90mg/mL