5D9A

Influenza C Virus RNA-dependent RNA Polymerase - Space group P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	107.280, 217.500, 597.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 4.300
R-factor	0.31839
Rwork	0.316
R-free	0.36847
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d98
RMSD bond length	0.009
RMSD bond angle	1.241
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0124)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.900	4.410
High resolution limit [Å]	4.300	4.300
Rmerge	0.204	0.993
Number of reflections	95264
<I/σ(I)>	5.5	1.1
Completeness [%]	99.0	93.1
Redundancy	3.3	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	Drops were set up by mixing polymerase (5 mg/ml in 25 mM Hepes:NaOH, pH 7.5, 10% (v/v) glycerol, 0.5 M NaCl, 0.5 mM TCEP, 10 mM CaCl2) with 0.2 M NaCl, 0.1 M Na:HEPES, pH 7.5, 25% (w/v) PEG4000 and microseeds in a 2:1:1 protein:precipitant:seed ratio.