5D91
Structure of a phosphatidylinositolphosphate (PIP) synthase from Renibacterium Salmoninarum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-09 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97911 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 48.632, 94.069, 103.916 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.976 - 2.501 |
R-factor | 0.2061 |
Rwork | 0.204 |
R-free | 0.24720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o6m |
RMSD bond length | 0.010 |
RMSD bond angle | 1.111 |
Data reduction software | XDS |
Data scaling software | Aimless (0.3.11) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 103.920 | 103.920 | 2.600 |
High resolution limit [Å] | 2.500 | 9.010 | 2.500 |
Rmerge | 0.271 | 0.081 | 1.716 |
Rpim | 0.063 | 0.022 | 0.467 |
Total number of observations | 320975 | 7516 | 21993 |
Number of reflections | 16926 | ||
<I/σ(I)> | 9.9 | 31.9 | 1.7 |
Completeness [%] | 98.2 | 99.9 | 84.5 |
Redundancy | 19 | 16.9 | 13.6 |
CC(1/2) | 0.999 | 0.999 | 0.636 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 20% (v/v) PEG 400, 0.1 M MES pH 6.7, 0.2 M lithium sulfate |