5D88
The Structure of the U32 Peptidase Mk0906
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-02 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 79.150, 79.150, 89.158 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.560 - 1.660 |
| R-factor | 0.133 |
| Rwork | 0.133 |
| R-free | 0.15200 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.8) |
| Refinement software | PHENIX (1.10-2131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.446 | |
| Number of reflections | 73564 | |
| <I/σ(I)> | 29.7 | 3.1 |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 5.1 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 2.5 M NaOAc |
